Nuclease Production and Lysostaphin Susceptibility of Staphylococcus aureus and Other Catalase-Positive Cocci

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Nuclease production and lysostaphin susceptibility of Staphylococcus aureus and other catalase-positive cocci.

Some strains of Staphylococcus epidermidis and Micrococcus sp. produce nucleases. However, thermal stability was shown to be unique to the nucleases of S. aureus. In addition, two micromethods for susceptibility testing to lysostaphin were more precise and convenient than anaerobic glucose fermentation in distinguishing between the genera Staphylococcus and Micrococcus.

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Lysostaphin-induced, osmotically fragile Staphylococcus aureus cells.

Staphylococcus aureus FDA 209P cells when suspended in 24% (w/v) NaCl were rendered osmotically fragile by exposure to lysostaphin for time intervals ranging from 2 to 60 min. Such cells were analyzed chemically and serologically for evidence of residual cell wall material, were plated in hypertonic sucrose medium to determine revertibility to normal, and were subjected to manometric studies to...

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The in vitro activity of lysostaphin against clinical isolates of Staphylococcus aureus was determined by conventional tube-dilution methods. For comparison, minimal inhibitory concentration (MIC) values were also determined for penicillin G, ampicillin, methicillin, ristocetin, vancomycin, and erythromycin. Phage type and penicillinase and coagulase production were determined for each isolate....

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1971

ISSN: 0003-6919

DOI: 10.1128/am.21.5.823-826.1971